False Negative

[ Quality Control/Quality Assurance Main | False Positives ]

Problem	Control Measures
Insufficient sensitivity of primer system	During design and validation: Computer aided primer design Test sensitivity of a new primer system in the single copy range on reference DNA of defined concentration
Inhibition of PCR reaction	During validation: Test primer system on sample DNA solutions known to contain inhibitory compounds During routine analysis: Test each PCR reaction for inhibition by an individual positive control (a spiked counterpart) Duplicate analysis of each sample
DNA extraction failed	Choose appropriate extraction protocol for sample type Run controls to monitor the efficiency of each series of extractions

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